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Purification of Trypanosoma cruzi surface proteins involved in adhesion to host cells
1Division of Biomedical Sciences, Meharry Medical College, Nashville, Tennessee 37208.
Biochemical and Biophysical Research Communications
|October 30, 1990
Abstract:
We have identified four surface 83 kDa proteins of pI values 6.3, 6.4, 6.5 and 6.6 in T. cruzi trypomastigotes which specifically bind to rat heart myoblasts. These proteins were purified by isoelectric focusing and anion-exchange chromatography in an FPLC system. These 83 kDa proteins inhibit the attachment of trypomastigotes to myoblasts in a concentration-dependent manner, indicating that these trypomastigote proteins mediate the attachment of trypomastigotes to heart myoblasts.