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[The use of antibodies against DNA modified by trans-diamminedichloroplatinum for identification of specific DNA

V I Kiseleva, M F Turchinskiĭ, A M Poverennyĭ

Bioorganicheskaia Khimiia

|July 1, 1990

Abstract:

DNA modified by trans-diamminedichloroplatinum (trans-DDP) is suggested as an effective probe for non-isotopic hybridisation. Highly specific anti trans-DDP-DNA antibodies and peroxidase-conjugated antibodies to antirabbit Ig and protein A system have been used for the hybrids visualisation. This method allows one to detect 2 pg/mm2 DNA.

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