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Updated: May 24, 2026
Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
Published on: June 14, 2024
A new loop-mediated isothermal amplification method for rapid, simple, and sensitive detection of Leptospira spp. in
Nobuo Koizumi1, Chie Nakajima, Tsunehito Harunari
1Department of Bacteriology, National Institute of Infectious Disease, Tokyo, Japan. nkoizumi@nih.go.jp
Abstract:
We developed a new loop-mediated isothermal amplification (LAMP) method to detect rrs, a 16S rRNA gene of pathogenic Leptospira spp. in urine. The method enables detection of two leptospiral cells per reaction mixture following boiling of urine specimens. The sensitivity of this method is higher than that of culture or of flaB nested PCR.
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