Related Experiment Video
Updated: May 24, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
Inhibitory RNA aptamer against SP6 RNA polymerase
Yusuke Mori1, Yoshikazu Nakamura, Shoji Ohuchi
1Department of Basic Medical Sciences, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Abstract:
Aptamers are attractive tools for modulating function of a desired target. In this study, we isolated an RNA aptamer that specifically inhibits transcription of SP6 RNA polymerase. The dissociation constant and 50% inhibitory concentration of the aptamer were estimated 9.5 nM and 24.8 nM, respectively. Doped-SELEX and mutational analysis revealed that the aptamer adopts the structure including two stems, two loops, and 5' single-stranded region. Based on the results, the aptamer could be engineered to circular permutant and binary construct forms without decreasing the activity. The aptamer would be applicable for the construction of expression regulation systems.
More Related Videos
Related Concept Videos
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Experimental RNAi
Eukaryotic RNA Polymerases
All three eukaryotic RNAPs require specific transcription factors, of which the...
Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Types of RNA
RNA Performs Diverse...

