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Updated: May 24, 2026
08:59
Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Streptomyces phospholipase D cloning and production
1Department of Food and Cosmetic Science, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan. y3nakaza@bioindustry.nodai.ac.jp
Methods in Molecular Biology (Clifton, N.J.)
|March 20, 2012
Summary
Phospholipase D (PLD) from actinomycetes efficiently synthesizes rare and artificial phospholipids. This enzyme accepts various alcohols, making it valuable for industries like pharmaceuticals and cosmetics.
Area of Science:
- Biochemistry
- Enzymology
- Microbiology
Background:
- Phospholipase D (PLD) catalyzes transphosphatidylation, a key reaction for synthesizing phospholipids.
- Actinomycete PLD exhibits broad substrate specificity, accepting diverse alcohols beyond natural ones.
- This enzymatic capability is crucial for producing rare and artificial phospholipids.
Purpose of the Study:
- To detail a comprehensive protocol for identifying and characterizing PLD-producing microorganisms.
- To establish methods for PLD assay, production, and purification.
- To outline a strategy for cloning the Streptomyces PLD gene.
Main Methods:
- Screening of microorganisms for PLD activity.
- Development and application of various PLD enzyme assays.
- Methods for microbial cultivation, enzyme purification, and gene cloning.
Main Results:
- Actinomycete PLD demonstrates broad substrate acceptance, including secondary alcohols, aromatic alcohols, saccharides, N-heterocyclic alcohols, and vitamins.
- Established protocols facilitate the production and purification of functional PLD.
- A strategy for cloning the relevant Streptomyces PLD gene was developed.
Conclusions:
- Actinomycete PLD is a versatile biocatalyst for generating diverse phospholipids.
- The described methods support the biotechnological application of PLD in various industries.
- Further research can leverage gene cloning for enhanced PLD engineering and application.
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