Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

3D pathology-guided microdissection.

Nature methods·2026
Same author

Systematic analysis of hippo pathway signaling identifies TEAD1 as a transcriptional regulator of neuroendocrine prostate cancer.

Neoplasia (New York, N.Y.)·2026
Same author

Integrative Surface Antigen Profiling of KLK2 and STEAP1 in Advanced Prostate Cancer.

Molecular cancer research : MCR·2026
Same author

Artificial Intelligence-informed Architectural Insights of 3-dimensional Glandular Networks Identify Patients With Prostate Cancer at a Higher Risk of Biochemical Recurrence.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
Same author

Characterization and Evaluation of CD24 and NPY as Biomarkers for Metastatic Castration-Resistant Prostate Cancer.

Diagnostics (Basel, Switzerland)·2026
Same author

Correction: Archaea express circular isoforms of IS200/IS605-associated ωRNAs.

Frontiers in microbiology·2026
Same journal

Correction to: Integrated morphological analyses of Cladomorphus phyllinus and transcriptomic analysis of Cladomorphus trimariensis provide insights into the cardiac morphophysiology of stick insects (Phasmida: Phasmatidae).

Cell and tissue research·2026
Same journal

Deletion of CEACAM1 does not affect retinal and choroidal morphology or transcriptome.

Cell and tissue research·2026
Same journal

Cardiac α2δ1 C-terminal contributes to left atrial hypertrophy in chronic ischemic heart failure, in association with changes in membrane GluN1 and p-CAMKII/p-HDAC4 signaling.

Cell and tissue research·2026
Same journal

Gill ionocytes of the Lake Magadi tilapia (Oreochromis Alcolapia grahami), an extremophilic teleost native to a highly alkaline environment.

Cell and tissue research·2026
Same journal

Integrated morphological analyses of Cladomorphus phyllinus and transcriptomic analysis of Cladomorphus trimariensis provide insights into the cardiac morphophysiology of stick insects (Phasmida: Phasmatidae).

Cell and tissue research·2026
Same journal

Effects of gestational protein restriction on autophagy dynamics during odontogenesis.

Cell and tissue research·2026
See all related articles

Related Experiment Video

Updated: May 24, 2026

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
06:01

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

Bladder expression of CD cell surface antigens and cell-type-specific transcriptomes.

Alvin Y Liu1, Ricardo Z N Vêncio, Laura S Page

  • 1Department of Urology and Institute for Stem Cell and Regenerative Medicine, University of Washington, Seattle, WA 98195, USA. aliu@uw.edu

Cell and Tissue Research
|March 20, 2012
PubMed
Summary
This summary is machine-generated.

Researchers compared gene expression in bladder and prostate cells to understand their functions. They found distinct gene expression patterns, suggesting altered stromal signaling in bladder cancer and offering new ways to study tissue development and cancer.

More Related Videos

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
11:02

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

Related Experiment Videos

Last Updated: May 24, 2026

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction
06:01

Expression of Transgenes in Native Bladder Urothelium Using Adenovirus-Mediated Transduction

Published on: October 6, 2022

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development
11:02

Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

Area of Science:

  • Urology
  • Cell Biology
  • Cancer Research

Background:

  • Basal epithelial and stromal cells in the bladder and prostate share similarities in appearance and cell surface markers.
  • Understanding the functional attributes of these similar-looking cells is crucial, especially in the context of cancer.
  • Urologic stromal cells are known to play a role in organ-specific tissue formation.

Purpose of the Study:

  • To compare the transcriptomes of various bladder and prostate cell types, including basal epithelial, stromal, urothelial, and cancer-associated cells.
  • To determine the functional relatedness of these cell types based on their gene expression profiles.
  • To investigate potential gene expression alterations in bladder cancer, particularly in stromal cells.

Main Methods:

  • Cell sorting using cluster designation (CD) antigens (e.g., CD9, CD104, CD13).
  • DNA microarray analysis to determine the transcriptome of sorted cell populations.
  • Principal components analysis (PCA) to compare transcriptome datasets and assess relatedness between cell types.

Main Results:

  • Bladder and prostate basal cells, despite sharing some CD markers, exhibited distinct overall gene expression profiles.
  • Basal cells did not show stem cell gene expression.
  • Transcriptomes of bladder luminal and stromal cells were different from their prostate counterparts.
  • In bladder cancer, both urothelial and stromal cells displayed altered gene expression.

Conclusions:

  • Gene expression analysis reveals functional differences between similar-looking bladder and prostate cell types.
  • Altered gene expression in stromal cells during bladder cancer suggests a potential role for defective stromal signaling in cancer development.
  • The generated cell-type transcriptomes can be utilized to develop and monitor in vitro models for studying bladder differentiation and tumor development.