Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Development of Immunocompetence01:22

Development of Immunocompetence

The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinically grounded retinal representation learning from minimal supervision.

Scientific reports·2026
Same author

A proof-of-concept automated method for accurate skin dosimetry: correcting overestimated surface dose measurements.

Physics in medicine and biology·2026
Same author

TopBP1 orchestrates PU.1-IRF8 transcriptional programming of dendritic cell differentiation and Flt3L-driven tumor immunity.

Experimental & molecular medicine·2026
Same author

Development and validation of an automated, accurate in-house treatment planning system for pencil-beam scanning carbon ion radiotherapy.

Medical physics·2026
Same author

Early fibrotic niches establish tumour-permissive microenvironments.

Nature·2026
Same author

Inhibiting Fatty Acid Oxidation Reverses Autophagy-Mediated Acquired Chemotherapy Resistance in Pancreatic Ductal Adenocarcinoma.

Cancer research·2026

Related Experiment Video

Updated: May 24, 2026

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

The SWI/SNF-like BAF complex is essential for early B cell development.

Jinwook Choi1, Myunggon Ko, Shin Jeon

  • 1Department of Biological Sciences, Institute of Molecular Biology and Genetics, Research Center for Functional Cellulomics, Seoul National University, Seoul 151-742, Korea.

Journal of Immunology (Baltimore, Md. : 1950)
|March 20, 2012
PubMed
Summary

The SWI/SNF-like BAF complex is crucial for early B cell development. Its subunits, Srg3/mBaf155 and Brg1, regulate key B lineage genes, ensuring proper cell progression.

More Related Videos

Studying Organelle Dynamics in B Cells During Immune Synapse Formation
15:39

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Published on: June 1, 2019

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice
05:03

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice

Published on: April 26, 2024

Related Experiment Videos

Last Updated: May 24, 2026

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

Studying Organelle Dynamics in B Cells During Immune Synapse Formation
15:39

Studying Organelle Dynamics in B Cells During Immune Synapse Formation

Published on: June 1, 2019

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice
05:03

Accessing Early Differentiation of Virus-Specific Follicular Helper CD4+ T Cell in Acute LCMV-Infected Mice

Published on: April 26, 2024

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Transcription factors like E2A and Ebf1 are vital for B cell development.
  • Chromatin regulators' roles in B lymphopoiesis are not well understood.
  • The SWI/SNF-like BAF complex is involved in gene expression regulation.

Purpose of the Study:

  • To investigate the role of SWI/SNF-like BAF complex subunits in early B cell development.
  • To determine if the SWI/SNF-like BAF complex regulates B lineage-specific genes.

Main Methods:

  • Hematopoietic lineage-specific deletion of Srg3/mBaf155.
  • Analysis of B cell development stages in knockout mice.
  • Assessing the expression of B lineage-specific genes (Ebf1, Il7ra) and downstream targets.

Main Results:

  • Deletion of Srg3/mBaf155 caused developmental defects at common lymphoid progenitor and pre-pro-B to early pro-B stages.
  • Deficiencies in Brg1 also impaired early B cell development.
  • The SWI/SNF-like BAF complex directly regulates Ebf1 and Il7ra expression.

Conclusions:

  • The SWI/SNF-like BAF complex is essential for early B cell development.
  • This complex facilitates B lymphopoiesis by controlling the expression of critical B lineage genes.