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Related Experiment Video

Updated: May 24, 2026

Rapid Development of Cell State Identification Circuits with Poly-Transfection
09:21

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Published on: February 24, 2023

Speed-dependent cellular decision making in nonequilibrium genetic circuits.

Nuno R Nené1, Jordi Garca-Ojalvo, Alexey Zaikin

  • 1Department of Mathematics, Imperial College London, London, United Kingdom. n.nene@imperial.ac.uk

Plos One
|March 20, 2012
PubMed
Summary

Cell fate decisions are influenced by signaling speed. Slow signal changes bias outcomes in gene regulatory circuits, while fast changes reduce this bias, revealing dynamic bifurcation importance.

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Area of Science:

  • * Systems biology and biophysics
  • * Nonlinear dynamics and gene regulatory networks

Background:

  • * Gene expression dynamics govern cell fate decisions, but the role of signaling speed remains unclear.
  • * Understanding nonequilibrium transitions is crucial for deciphering cellular decision-making processes.

Purpose of the Study:

  • * To investigate how the speed of external signals affects cell fate decisions in a symmetric gene regulatory circuit.
  • * To determine if transient signaling asymmetry can bias cellular outcomes.

Main Methods:

  • * Modeling a gene circuit with two mutually inhibiting, self-activating genes.
  • * Applying two external signals with identical steady states but different transient times.
  • * Analyzing the system's response to slow versus fast passage through a decision point.

Main Results:

  • * Slow passage through the decision point leads to consistently biased cell fate decisions due to transient signaling asymmetry.
  • * Fast passage reduces and eventually eliminates this decision bias.
  • * The observed bias is robust to noise.

Conclusions:

  • * Signaling speed is a critical factor in biasing cell fate decisions.
  • * Dynamic bifurcations, a concept from nonequilibrium physics, play a significant role in controlling genetic circuits.
  • * This finding offers insights into the fundamental mechanisms of cellular decision-making.