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Updated: May 23, 2026
3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer
Published on: September 13, 2018
Biomarker research and some deduction in superficial bladder cancer cells combined with corresponding stroma
Hai Tao Niu1, Chuan Min Yang, Bo Chen
1Department of Urology, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong, China. Niuhaitao1976@gmail.com
Aims:
To facilitate biomarker discovery and improve cancer biology research of superficial bladder transitional cell carcinoma (SBTCC).
Materials And Methods:
Laser capture microdissection and two-dimensional liquid chromatography tandem mass spectrometry was used to identified the proteome expression profile of purified normal urothelial cells (cancer cells)/normal stromal cells (cancer stromal cells). Based on the expression profile, biomarker discovery and the mechanisms of multi-step carcinogenesis were discussed.
Results:
775/792 proteins commonly appeared in 4 paired cancer/normal tissue. 339 differential proteins located at the well-known biological KEGG pathways and defined as potential biomarkers. 53/31 proteins specific repeat expression in cancer tissue/normal tissue and thought to play important role in tumor--stroma interactions.
Conclusion:
Proteins origin from cancer or stromal cells have the same probability to be a biomarker. The significant altered pathways mainly include metabolic pathways, spliceosome, endocytosis, oxidative phosphorylation, etc. Most of the specific repeat expression proteins act important role in cancer biology.
