[The effect of adiponectin on human periodontal ligament fibroblasts in vitro]

To investigate the expression of adiponectin receptor in periodontal tissues and the effect of adiponectin with different concentrations on the proliferation of human periodontal ligament fibroblast cells.

Ten human healthy premolars extracted for orthodontic reasons were collected. The primary cells were isolated from human periodontal ligament and cultured by tissue block method,then passaged when the cells completely covered the bottom of petri dish. The expression of AdipoR1 and AdipoR2 in human periodontal ligament fibroblasts was detected by RT-PCR. Adiponectin (0,5,10,20ng/μL) was added to petri dish in which the cells were incubated for 24 hours.The cells were then incubated with adiponectin for 7 days,and determined by MTT assay.SPSS16.0 software package was used for statistical analysis.

By immunocytochemical method,the cells were stained positively to antibodies against vimentin,and negatively to antibodies against cytokeratin,indicating that they were external embryo mesenchymal cells ,without epithelial cell mixation. Agarose gel electrophoresis showed Adipo R2 was positively expressed in human periodontal ligament fibroblasts. MTT assay showed that proper doses of adiponectin could promote proliferation of human periodontal ligament fibroblasts (P<0.05).The number of adherent cells of group C or group D was significantly greater than that of group A after 24 hours(P<0.05); Group B, group C,and group D had significantly more adherent cells than group A after 96 hours and 168 hours (P<0.05).

Adiponectin receptor 2 is expressed in the periodontal tissues.The proper doses of adiponectin could promote proliferation of human periodontal ligament fibroblasts.