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Updated: May 23, 2026
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Published on: May 21, 2015
[Using DGGE to analyze bacteria community structure changes in subgingival plaque after periodontal initial therapy]
Yan-bin Zhou1, Rong Shu, Da-li Liu
1Department of Periodontology, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Purpose:
To analyze bacteria community structure changes in subgingival plaque after initial therapy, and to provide experimental evidences for clinical decision.
Methods:
Six patients with chronic periodontitis were chosen. Subgingival plaque samples, as well as clinical indexes, were collected at baseline and six weeks after initial therapy. The generated two different 16S rDNA fragments of subgingival plaque samples were separated by denaturing gel, creating bands patterns representative of community structure. Subsequent cluster analysis was made.
Results:
The clinical indexes were improved significantly. The diversity of population of clinical subgingival samples was not significantly different between baseline and 6 weeks after initial therapy. Through cluster analysis, it was confirmed that same patient got similar subgingival plaque between baseline and 6 weeks after treatment.
Conclusions:
Same patient tend to get similar subgingival plaque between baseline and after initial therapy. DGGE can detect the microbial composition of subgingival plaque.
