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Updated: May 23, 2026

Strategies for Assessing Autistic-Like Behaviors in Mice
Published on: September 20, 2024
Autism gene variant causes hyperserotonemia, serotonin receptor hypersensitivity, social impairment and repetitive
Jeremy Veenstra-VanderWeele1, Christopher L Muller, Hideki Iwamoto
1Department of Psychiatry, Vanderbilt University, Nashville, TN 37232, USA. j.vvw@vanderbilt.edu
Abstract:
Fifty years ago, increased whole-blood serotonin levels, or hyperserotonemia, first linked disrupted 5-HT homeostasis to Autism Spectrum Disorders (ASDs). The 5-HT transporter (SERT) gene (SLC6A4) has been associated with whole blood 5-HT levels and ASD susceptibility. Previously, we identified multiple gain-of-function SERT coding variants in children with ASD. Here we establish that transgenic mice expressing the most common of these variants, SERT Ala56, exhibit elevated, p38 MAPK-dependent transporter phosphorylation, enhanced 5-HT clearance rates and hyperserotonemia. These effects are accompanied by altered basal firing of raphe 5-HT neurons, as well as 5HT(1A) and 5HT(2A) receptor hypersensitivity. Strikingly, SERT Ala56 mice display alterations in social function, communication, and repetitive behavior. Our efforts provide strong support for the hypothesis that altered 5-HT homeostasis can impact risk for ASD traits and provide a model with construct and face validity that can support further analysis of ASD mechanisms and potentially novel treatments.
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