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Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
Published on: October 20, 2011
Microbial community succession on developing lesions on human enamel
Lino Torlakovic1, Vanja Klepac-Ceraj, Bjørn Ogaard
1Department of Oral Biology, University of Oslo, Oslo, Norway.
Journal of Oral Microbiology
|March 21, 2012
Summary
Dental caries progression involves specific microbial shifts. This study used an in vivo model to identify bacterial taxa associated with early enamel lesions, revealing a complex oral microbiome dynamic.
Area of Science:
- Oral microbiology
- Dental research
- Microbial ecology
Background:
- Dental caries is a prevalent global disease.
- Understanding in vivo microbial shifts during caries development is limited.
- Longitudinal studies are crucial for elucidating caries progression.
Purpose of the Study:
- To investigate microbial community composition changes during enamel caries development.
- To utilize an in vivo longitudinal model for studying caries.
- To identify specific bacterial taxa associated with early enamel lesions.
Main Methods:
- Generated white spot lesions in vivo on human teeth.
- Analyzed bacterial microbiota using the Human Oral Microbe Identification Microarray (HOMIM).
- Compared microbial communities on sound enamel versus developing carious lesions.
Main Results:
- 75% of teeth developed lesions within seven weeks.
- Significant differences in microbial composition between lesion and sound enamel plaque.
- Identified specific bacterial taxa, including Streptococcus mutans, associated with initial enamel lesions.
Conclusions:
- The bacterial community associated with enamel lesion progression is specific and complex.
- This longitudinal in vivo model is effective for studying supragingival microbial succession in caries.
- Findings support previous cross-sectional data and offer a model for treatment efficacy studies.
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