Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Membrane Fluidity01:23

Membrane Fluidity

Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ku70-SAP domain has an overlapping function with DNA-PKcs in limiting the lateral movement of the Ku ring along DNA.

Nucleic acids research·2026
Same author

SmartTrap: automated precision experiments with optical tweezers.

Nature methods·2026
Same author

A platinum butterfly effect: small changes turn an anticancer drug into a non-toxic metalloantibiotic with in vivo efficacy.

npj antimicrobials and resistance·2026
Same author

Size-based sorting of cancer cells reveals functional heterogeneity among subpopulations.

Lab on a chip·2026
Same author

CRL2<sup>FEM1B</sup> uses heme to recruit BACH1 for degradation and regulate ferroptosis in lung cancer.

Molecular cell·2026
Same author

Using Bayes' Rule for Analysis of Microfluidic Particle and Cluster Sorting.

Micromachines·2026

Related Experiment Video

Updated: May 23, 2026

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Lipid-based passivation in nanofluidics.

Fredrik Persson1, Joachim Fritzsche, Kalim U Mir

  • 1Department of Physics, University of Gothenburg, Gothenburg, Sweden.

Nano Letters
|March 22, 2012
PubMed
Summary
This summary is machine-generated.

Researchers developed a lipid bilayer passivation method for nanochannels, significantly improving studies of DNA-protein interactions. This technique enables long-term, high-resolution monitoring of single DNA molecules and their interactions with proteins.

More Related Videos

Surface Passivation for Single-molecule Protein Studies
10:35

Surface Passivation for Single-molecule Protein Studies

Published on: April 24, 2014

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Related Experiment Videos

Last Updated: May 23, 2026

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

Surface Passivation for Single-molecule Protein Studies
10:35

Surface Passivation for Single-molecule Protein Studies

Published on: April 24, 2014

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles
11:13

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

Area of Science:

  • Molecular Biology
  • Biophysics
  • Nanotechnology

Background:

  • Stretching DNA in nanochannels allows single-molecule studies of genomic material.
  • Studying protein-DNA interactions in nanochannels requires effective surface passivation to prevent non-specific binding.

Purpose of the Study:

  • To implement and evaluate a lipid bilayer-based passivation scheme for nanochannels.
  • To enhance the study of linear DNA and protein interactions at the single-molecule level.
  • To enable long-term, high-resolution monitoring of protein-DNA interactions.

Main Methods:

  • Developed a lipid bilayer passivation technique for nanochannel surfaces.
  • Tested passivation effectiveness against streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes.
  • Compared lipid bilayer performance to standard bovine serum albumin (BSA) passivation.
  • Monitored single DNA cleavage events using DNase I on passivated devices.

Main Results:

  • Achieved virtually complete, long-term passivation of nanochannel surfaces.
  • Demonstrated superior performance of lipid bilayers compared to BSA passivation.
  • Successfully monitored individual DNA cleavage events in real-time.

Conclusions:

  • Lipid bilayer passivation is a highly effective method for nanochannel applications.
  • This approach significantly improves the study of protein-DNA interactions with high spatial and temporal resolution.
  • The developed technique facilitates detailed, systematic investigations of molecular mechanisms.