Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Mutations01:39

Mutations

Overview
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unraveling the Therapeutic Mechanisms of Aloe-Emodin: A Natural Anthraquinone With Promising Health Benefits.

Archiv der Pharmazie·2026
Same author

Unveiling the anti-inflammatory activity of chloroform fraction of curcuma wallichii and its phytoconstituents by in vivo and in silico studies.

Scientific reports·2026
Same author

Unveiling the apoptotic potential of antioxidant-rich Bangladeshi medicinal plant extractives and computational modeling to identify antitumor compounds.

Heliyon·2024
Same author

Experimental and pharmacoinformatic approaches unveil the neuropharmacological and analgesic potential of chloroform fraction of Roktoshirinchi (Achyranthes ferruginea Roxb.).

Journal of ethnopharmacology·2024
Same author

Exploring the therapeutic potential of edible vegetables, fruits, and spices against cancer in various cell lines.

Journal of Cancer·2024
Same author

Apoptosis-inducing anti-proliferative and quantitative phytochemical profiling with in silico study of antioxidant-rich <i>Leea aequata</i> L. leaves.

Heliyon·2024

Related Experiment Video

Updated: May 23, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Alternative splicing produces structural and functional changes in CUGBP2.

Hitoshi Suzuki1, Makoto Takeuchi, Ayumu Sugiyama

  • 1Center for Nano Materials and Technology, Japan Advanced Institute of Science and Technology, Ishikawa 923-1292, Japan. suzuki-h@jaist.ac.jp

BMC Biochemistry
|March 22, 2012
PubMed
Summary

Alternative splicing of CUGBP2 exon 14 impacts gene regulation. Structural changes in CUGBP2 isoforms alter biological activity, affecting splicing of ACTN1 and insulin receptor genes.

More Related Videos

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Related Experiment Videos

Last Updated: May 23, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

Area of Science:

  • Molecular Biology
  • RNA Biology
  • Protein Structure

Background:

  • CELF/Bruno-like proteins, including CUGBP2, are crucial RNA-binding proteins involved in alternative splicing and translation.
  • CUGBP2 features multiple RNA recognition motif (RRM) domains and undergoes alternative splicing.
  • Specific focus on CUGBP2 exon 14, encoding part of the third RRM.

Purpose of the Study:

  • Investigate the expression and functional impact of CUGBP2 exon 14 alternative splicing.
  • Determine how different CUGBP2 isoforms affect the splicing of target genes.
  • Elucidate the structural basis for functional differences between CUGBP2 isoforms.

Main Methods:

  • Analysis of exon 14 skipping in neuronal cells and brain tissue.
  • Assessing the impact of CUGBP2 isoforms on ACTN1 and insulin receptor gene splicing.
  • Employing molecular dynamics simulation and NMR spectrometry to study protein structure.

Main Results:

  • Reduced exon 14 skipping in neuronal cells and brain.
  • CUGBP2 isoforms differentially regulate ACTN1 and insulin receptor splicing.
  • The R3δ isoform's third RRM exhibits flexibility, lacking a stable structure.

Conclusions:

  • CUGBP2 employs distinct mechanisms to regulate ACTN1 and insulin receptor splicing.
  • Alternative splicing of CUGBP2 exon 14 is key to insulin receptor splicing regulation.
  • Alternative splicing-induced structural changes in CUGBP2 modulate its biological activity.