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[Quantitative analyses of intrahepatic HBV cccDNA and serum HBsAg in 54 patients with chronic hepatitis B]

Wei-jie Li1, Bo-an Li, Jing-min Zhao

  • 1Liver Failure Management and Research Center, the Military 302 Hospital, Beijing 100039, China.

Zhonghua Gan Zang Bing Za Zhi = Zhonghua Ganzangbing Zazhi = Chinese Journal of Hepatology
|March 22, 2012
PubMed

Insights

In chronic hepatitis B patients, intrahepatic hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) correlates with serum HBsAg. These markers, with serum HBV DNA, may assess viral activity and treatment effectiveness.

Area of Science:

  • Hepatology
  • Virology
  • Molecular Biology

Context:

  • Chronic hepatitis B (CHB) is a significant global health concern.
  • Accurate assessment of intrahepatic viral activity is crucial for managing CHB.
  • Current methods for quantifying intrahepatic viral markers have limitations.

Purpose:

  • To quantitatively measure intrahepatic hepatitis B virus covalently closed circular DNA (cccDNA) and serum HBsAg.
  • To analyze the correlation between intrahepatic cccDNA, serum HBsAg, and serum HBV DNA levels.
  • To explore the utility of these markers in reflecting HBV replication and guiding antiviral therapy.

Summary:

  • A novel method combining PSAD digestion, rolling circle amplification, and real-time PCR was used to quantify intrahepatic cccDNA from liver biopsies.
  • Intrahepatic cccDNA levels showed a positive correlation with serum HBsAg (r = 0.459, P < 0.001).
  • Serum HBsAg also correlated positively with serum HBV DNA (r = 0.328, P = 0.015) and inversely with HBV replicative efficiency (r = -0.373, P = 0.005).

Impact:

  • This study establishes a quantitative link between intrahepatic cccDNA and serum HBsAg in CHB patients.
  • Combined assessment of intrahepatic cccDNA, serum HBsAg, and serum HBV DNA may offer a comprehensive evaluation of HBV replication.
  • These findings could enhance the monitoring of antiviral treatment efficacy in CHB management.
Abstract

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