Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A direct method for fast three-dimensional serial reconstruction.

A Odgaard1, K Andersen, F Melsen

  • 1Biomechanics Laboratory, Orthopaedic Hospital, University of Aarhus, Denmark.

Journal of Microscopy
|September 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patient experiences after surgically treated periprosthetic knee fracture in the distal femur - an explorative qualitative study.

International journal of orthopaedic and trauma nursing·2025
Same author

A literature review and expert consensus statement on diagnostics in suspected metal implant allergy.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Mismatch 'never events' in hip and knee arthroplasty: a cohort and intervention study.

The bone & joint journal·2019
Same author

Re-admissions, re-operations and length of stay in hospital after aseptic revision knee replacement in Denmark: a two-year nationwide study.

The bone & joint journal·2014
Same author

Translation, cross-cultural adaptation and validation of the Danish version of the Oxford hip score: Assessed against generic and disease-specific questionnaires.

Bone & joint research·2013
Same author

Local infiltration analgesia for total knee arthroplasty: should ketorolac be added?

British journal of anaesthesia·2013
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

This study presents a new method for 3D reconstruction of porous structures using epoxy resin embedding and serial sectioning. The automated process efficiently digitizes and stores images for detailed analysis of complex materials like trabecular bone.

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Microscopy

Background:

  • Accurate 3D reconstruction of porous structures is crucial for understanding material properties.
  • Existing methods may lack efficiency or precision for complex architectures.
  • Trabecular bone presents a challenging anisotropic porous structure for analysis.

Purpose of the Study:

  • To develop and validate a novel, automated method for high-resolution 3D reconstruction of openly connected porous structures.
  • To demonstrate the method's applicability to complex biological tissues, such as trabecular bone.

Main Methods:

  • Specimen embedding in contrast-colored epoxy resin.
  • Serial sectioning using a hard tissue microtome.
  • PC-based image processing for direct digitization and thresholding to obtain two-phase images.

Related Experiment Videos

Main Results:

  • The method enables accurate three-dimensional reconstruction of porous materials.
  • The process is fully automated, achieving high throughput (approx. 170 sections/hour).
  • Successful application demonstrated on anisotropic trabecular bone structures.

Conclusions:

  • This automated serial sectioning and imaging method provides an efficient and accurate approach for 3D reconstruction of porous structures.
  • The technique is suitable for detailed analysis of complex microarchitectures, including trabecular bone.