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Imaging of HIV-1 Envelope-induced Virological Synapse and Signaling on Synthetic Lipid Bilayers
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Changes in the transmembrane region of the human immunodeficiency virus type 1 gp41 envelope glycoprotein affect

E Helseth1, U Olshevsky, D Gabuzda

  • 1Dana-Farber Cancer Institute, Department of Pathology, Harvard Medical School, Boston, Massachusetts.

Journal of Virology
|December 1, 1990
PubMed

The charged amino acids near or within the membrane-spanning region of the human immunodeficiency virus type 1 gp41 envelope glycoprotein were altered. Two mutants were defective for syncytium formation and virus replication even though levels of envelope glycoproteins on the cell or virion surface and CD4 binding were comparable to those of the wild-type proteins. Thus, in addition to anchoring the envelope glycoproteins, sequences proximal to the membrane-spanning gp41 region are important for the membrane fusion process.

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