Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid zeolitic imidazolate frameworks with catalytically active TO4 building blocks.

Angewandte Chemie (International ed. in English)·2010
Same author

Whiter matter abnormalities in medication-naive subjects with a single short-duration episode of major depressive disorder.

Psychiatry research·2010
Same author

A new comorbidity index: the health-related quality of life comorbidity index.

Journal of clinical epidemiology·2010
Same author

S-adenosylmethionine inhibits the growth of cancer cells by reversing the hypomethylation status of c-myc and H-ras in human gastric cancer and colon cancer.

International journal of biological sciences·2010
Same author

Nano-sized SnSbAgx alloy anodes prepared by reductive co-precipitation method used as lithium-ion battery materials.

Journal of nanoscience and nanotechnology·2010
Same author

Complementary diffusion tensor imaging study of the corpus callosum in patients with first-episode and chronic schizophrenia.

Journal of psychiatry & neuroscience : JPN·2010
Same journal

MST4 Regulates Microglia Neuroinflammation via Targeting PKM2 Nuclear Translocation in Epilepsy: An In Vivo and In Vitro Study.

Neurochemical research·2026
Same journal

Correction to: Dual Antioxidant DH-217 Mitigated Cerebral Ischemia-Reperfusion Injury by Targeting IKKβ/Nrf2/HO-1 Signal Axis.

Neurochemical research·2026
Same journal

Microglia-Dependent BDNF Signaling in the Dentate Gyrus Underlies the Antidepressant Effects of Gardiquimod, a Toll-Like Receptor 7 Agonist, in Chronically Stressed Mice.

Neurochemical research·2026
Same journal

Cryptotanshinone Targets Ferroptosis in Glioma via the EGFR/ROS Signaling Pathway.

Neurochemical research·2026
Same journal

Combined Puerarin and Magnesium Acetyl Taurate Intervention Mitigates Autism-Like Pathology Through Glutamatergic and MAPK Pathway Regulation.

Neurochemical research·2026
Same journal

Long-Term Impact of Early-Life Stress on Hippocampal Apoptotic Gene Expression in BALB/c Mice.

Neurochemical research·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
09:30

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

Efficient gene transfer into neonatal mouse brain using electroporation.

Xue-Feng Ding1, Yong-Qi Zhao, Zeng-Yao Hu

  • 1Department of Cognitive science, Beijing Institute of Basic Medical Sciences, Beijing, 100850, People's Republic of China.

Neurochemical Research
|March 22, 2012
PubMed
Summary
This summary is machine-generated.

This study presents a simplified, non-surgical in vivo electroporation technique for efficient gene transfer in rodent brains. The improved method enhances gene expression and survival rates in neonates, aiding neuroscience research.

More Related Videos

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
13:12

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Published on: June 23, 2011

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
10:15

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Published on: September 15, 2014

Related Experiment Videos

Last Updated: May 23, 2026

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection
09:30

Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection

Published on: August 15, 2011

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
13:12

Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation

Published on: June 23, 2011

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction
10:15

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Published on: September 15, 2014

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Gene Therapy

Background:

  • In vivo electroporation is a key technique for gene transfer in the rodent brain.
  • Existing methods involve invasive surgery and have limitations in efficiency and complexity.

Purpose of the Study:

  • To develop a simplified and more efficient in vivo electroporation method for gene transfer into the postnatal rodent forebrain.
  • To reduce trauma and improve transfection success rates in newborn rodents.

Main Methods:

  • Modified electroporation protocol with decreased pulse voltage (90 V).
  • Non-surgical DNA injection into the forebrain of neonatal pups.
  • Assessment of survival rates, transfection efficiency, and duration of gene expression.

Main Results:

  • DNA injection completed within 30 seconds without surgery.
  • Survival rate exceeding 94% in injected neonates.
  • Gene expression observed in nearly 100% of survivors, persisting for up to 20 days.

Conclusions:

  • The improved non-surgical electroporation method is simple, efficient, and safe for gene transfer in postnatal rodent brains.
  • This technique provides a powerful tool for studying gene function in postnatal neurogenesis and neural development.