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Updated: May 23, 2026

A Versatile Method of Patterning Proteins and Cells
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A Versatile Method of Patterning Proteins and Cells

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Micro-patterned porous substrates for cell-based assays.

Fanny Evenou1, Jean-Marc Di Meglio, Benoit Ladoux

  • 1Matière et Systèmes Complexes, UMR 7057 CNRS & Université Paris Diderot, 75013 Paris, France.

Lab on a Chip
|March 22, 2012
PubMed
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This summary is machine-generated.

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Researchers developed Micro-Printed Membranes (μPM), a novel lab-on-chip system for efficient cell-based assays. This versatile device enables precise chemical delivery and screening of multiple compounds with low sample volumes.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Lab-on-a-chip systems offer efficient platforms for cell-based assays and high-throughput screening.
  • Novel tools are needed to advance drug discovery and chemical testing.
  • Spatial control of chemical exposure in cell cultures is crucial for accurate assay results.

Purpose of the Study:

  • To introduce a novel, versatile, and simple lab-on-chip device for bench-top cell-based assays.
  • To demonstrate the capability of Micro-Printed Membranes (μPM) for spatially controlled chemical stimulation of cells.
  • To validate the potential of μPM for high-throughput screening and drug discovery.

Main Methods:

  • Fabrication of Micro-Printed Membranes (μPM) by microcontact printing of polydimethylsiloxane (PDMS) on membrane filters to create spatial porosity variations.

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Last Updated: May 23, 2026

A Versatile Method of Patterning Proteins and Cells
09:57

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Published on: February 26, 2017

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates
07:19

Cell Patterning on Photolithographically Defined Parylene-C: SiO2 Substrates

Published on: March 7, 2014

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09:30

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

  • Dispensing active compounds onto porous areas of the μPM to allow chemical diffusion to cells cultured on the opposite side.
  • Proof-of-principle experiments using Hoechst nuclear staining, calcein-AM cell viability assay, and cytochalasin B treatment to assess cellular response.

    • Main Results:

    • Fluorescent staining results accurately corresponded to the positioning of dispensed compounds, confirming spatial control.
    • No cross-contamination was observed between adjacent test areas, indicating assay specificity.
    • The μPM system demonstrated successful cell stimulation only in regions directly below porous areas.

    Conclusions:

    • Micro-Printed Membranes (μPM) provide a flexible, well-less system for cell-based assays requiring low sample volumes.
    • The device enables sequential and combinatorial drug assays with real-time cell monitoring.
    • μPM technology is well-suited for adaptation to both manual and automated high-content screening platforms.