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Flow cytometric DNA analysis of thyroid carcinoma
A Tsuchiya1, K Sekikawa, Y Ando
1Second Department of Surgery, Fukushima Medical College, Japan.
The Japanese Journal of Surgery
|September 1, 1990
Summary
Flowcytometric DNA analysis in thyroid carcinoma revealed that aneuploidy, or abnormal DNA content, is linked to decreased survival rates. While not a perfect predictor, DNA content aids in assessing tumor behavior and patient prognosis.
Area of Science:
- Oncology
- Molecular Biology
- Pathology
Background:
- Abnormal DNA content is a potential marker for tumor biological behavior.
- Flow cytometry is a technique used to analyze DNA content in tumor cells.
Purpose of the Study:
- To investigate the incidence of aneuploidy in various types of thyroid carcinoma.
- To evaluate the correlation between DNA content, S-phase fraction, and patient survival in thyroid carcinoma.
Main Methods:
- Flowcytometric DNA analysis was performed on 121 thyroid carcinoma tissue samples from paraffin-embedded blocks.
- Histological classification included papillary, follicular, medullary, squamous cell, and anaplastic carcinomas.
- S-phase fraction was determined as a measure of proliferative potential.
Main Results:
- Aneuploidy was observed in 7.4% of thyroid carcinoma cases, with diploidy in 92.6%.
- Aneuploid specimens were found in papillary, follicular, medullary, and anaplastic carcinomas.
- Aneuploid patients exhibited a significantly decreased survival rate (p < 0.01).
- S-phase fraction was not found to be an independent prognostic factor.
Conclusions:
- DNA content analysis is useful for predicting the survival of aneuploid thyroid carcinoma patients.
- There is a correlation between DNA content and tumor biological behavior, though some discrepancies exist.
- Aneuploidy serves as an additional criterion for assessing the prognosis of thyroid carcinoma.