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Updated: May 23, 2026
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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
Published on: March 17, 2023
Increasing throughput of surface plasmon resonance-based biosensors by multiple analyte injections
Massinissa Si Mehand1, Gregory De Crescenzo, Bala Srinivasan
1Department of Chemical Engineering, École Polytechnique de Montréal, PO Box 6079, Centre-ville Station, Montréal, Québec, Canada H3C 3A7.
Journal of Molecular Recognition : JMR
|March 22, 2012
Summary
This study optimizes surface plasmon resonance (SPR) assays for faster biomolecular interaction analysis. Combining iterative optimization with simultaneous dual-analyte injection significantly reduces experiment time for kinetic parameter determination.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biophysics
Background:
- Surface plasmon resonance (SPR) biosensors are vital for determining biomolecular interaction kinetics.
- Reducing experimental time in SPR assays is crucial for improving screening campaign throughput.
- Current methods involve analyzing multiple analyte injections at varying concentrations.
Purpose of the Study:
- To explore reducing SPR experiment duration further.
- To investigate combining iterative optimization with simultaneous dual-analyte injection.
- To assess the impact on kinetic parameter determination accuracy.
Main Methods:
- Utilizing an iterative optimization approach for online analyte injection selection.
- Implementing simultaneous injection of two analytes in SPR assays.
- Analyzing kinetic parameters derived from optimized experimental designs.
Main Results:
- The combined approach significantly reduces experiment time for kinetic parameter determination.
- No detrimental effect on the standard errors of kinetic parameters was observed.
- Demonstrated feasibility of accelerating SPR-based kinetic analysis.
Conclusions:
- Iterative optimization combined with simultaneous dual-analyte injection is an effective strategy for accelerating SPR assays.
- This method enhances throughput in biomolecular interaction studies.
- Further research can explore broader applications of this accelerated kinetic analysis technique.
