Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Bioremediation of Plastics01:28

Microbial Bioremediation of Plastics

Polyethylene terephthalate (PET) is a synthetic polymer widely utilized in the packaging industry, particularly for bottles and containers. Due to its chemical stability and durability, PET accumulates in the environment, contributing significantly to plastic pollution. It comprises repeating units of terephthalic acid and ethylene glycol, resulting in a semi-crystalline structure that is resistant to natural degradation processes.A notable breakthrough in plastic biodegradation came with the...
Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Assessment of Endoscope Tip Angulation Performance in New Endoscopes: A Cross-Sectional Quality Improvement Study.

Endoscopy international open·2026
Same author

Dynamic progression of ectopic lymphoid structure formation in lacrimal glands of a Sjögren's disease murine model.

Frontiers in immunology·2026
Same author

AI-validated fusion proteins for local inhibition of interleukin-17A.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Urologic Device Innovation and Trends: An Analysis of Food and Drug Administration Approvals From 1990 to 2023.

Urology practice·2026
Same author

Real-world Utilization of Mineralocorticoid Receptor Antagonists in India and the Benefits of GDMT in Heart Failure.

The Journal of the Association of Physicians of India·2026
Same author

Budesonide-glycopyrronium-formoterol fumarate dihydrate in uncontrolled asthma (KALOS and LOGOS): twin multicentre, double-blind, double-dummy, parallel-group, randomised, phase 3 trials.

The Lancet. Respiratory medicine·2026

Related Experiment Video

Updated: May 23, 2026

Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method
06:30

Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method

Published on: January 9, 2026

Biodegradation of elastin-like polypeptide nanoparticles.

Mihir Shah1, Pang-Yu Hsueh, Guoyong Sun

  • 1Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90033-9121, USA.

Protein Science : a Publication of the Protein Society
|March 22, 2012
PubMed
Summary
This summary is machine-generated.

Protein polymers like Elastin-like polypeptides (ELPs) can be degraded by proteases, even within nanoparticles. This biodegradation is crucial for developing ELPs as biopharmaceuticals for drug delivery.

More Related Videos

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
12:48

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Published on: December 27, 2013

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Related Experiment Videos

Last Updated: May 23, 2026

Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method
06:30

Efficient Purification of Elastin-Like Polypeptides (ELPs) from E. coli Using an Organic Solvent-based Extraction and Precipitation Method

Published on: January 9, 2026

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS
12:48

PLGA Nanoparticles Formed by Single- or Double-emulsion with Vitamin E-TPGS

Published on: December 27, 2013

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Protein polymers, such as Elastin-like polypeptides (ELPs), are synthesized via ribosomal pathways and show promise for biopharmaceutical applications.
  • Biodegradation is a critical factor for the in vivo application of protein polymers.
  • ELPs exhibit a unique inverse phase transition temperature (T(t)), enabling reversible solubility changes and potential for controlled drug delivery.

Purpose of the Study:

  • To characterize the proteolytic biodegradation of ELP nanoparticles.
  • To investigate the susceptibility of ELP nanoparticles to proteases after cellular uptake.
  • To understand the influence of the ELP phase transition on protease activity.

Main Methods:

  • Utilized a library of ELPs derived from human tropoelastin.
  • Assayed proteolytic degradation using two model proteases: elastase and collagenase.
  • Investigated proteolysis in both soluble ELPs and ELP nanoparticles, including after uptake into murine hepatocytes.

Main Results:

  • ELP nanoparticles are degradable by both elastase and collagenase.
  • Comparable proteolysis of ELP nanoparticles was observed after uptake into hepatocytes.
  • The ELP phase transition provided protection against collagenase but not elastase activity.

Conclusions:

  • ELP nanoparticles are susceptible to proteolytic degradation by common endopeptidases.
  • The phase transition behavior of ELPs influences their susceptibility to specific proteases.
  • These findings are essential for predicting ELP biodegradation in physiological environments and advancing their use as biopharmaceuticals.