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Pull-down of Calmodulin-binding Proteins
07:51

Pull-down of Calmodulin-binding Proteins

Published on: January 23, 2012

Three functional facets of calbindin D-28k.

Hartmut Schmidt1

  • 1Medical Faculty, Carl-Ludwig Institute for Physiology, University of Leipzig Leipzig, Germany. hartmut.schmidt@medizin.uni-leipzig.de

Frontiers in Molecular Neuroscience
|March 22, 2012
PubMed
Summary

Calbindin D-28k (CB) in the central nervous system (CNS) acts as a calcium buffer, transporter, and a non-canonical sensor. This protein influences synaptic calcium dynamics, impacting neuronal plasticity and information processing.

Keywords:
buffercalciumneuronssensorsynaptic plasticitytransmitter releasetransporter

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Calbindin D-28k (CB) is a Ca(2+) binding protein found in many vertebrate central nervous system (CNS) neurons, including Purkinje cells and neocortical interneurons.
  • Traditionally recognized for its role as a Ca(2+) buffer crucial for neuronal survival, CB's functions have been re-evaluated.

Purpose of the Study:

  • To explore the multifaceted roles of calbindin D-28k (CB) beyond its classical function as a calcium buffer.
  • To investigate CB's involvement in synaptic calcium dynamics, neuronal plasticity, and information processing.
  • To examine the potential of CB acting as a calcium sensor.

Main Methods:

  • Literature review and synthesis of existing research on calbindin D-28k (CB).
  • Analysis of studies investigating CB's interaction with target proteins.
  • Examination of evidence for CB's role in calcium transport.

Main Results:

  • CB exhibits moderate cytoplasmic mobility and possesses EF-hands for Ca(2+) binding with varying kinetics and affinity.
  • CB critically influences synaptic Ca(2+) dynamics, suggesting a role in plasticity and information processing.
  • Evidence supports CB's function as a Ca(2+) sensor, interacting with targets in both Ca(2+)-bound and Ca(2+)-free states.
  • CB is implicated in buffered Ca(2+) transport in neurons and kidney.

Conclusions:

  • Calbindin D-28k (CB) serves a threefold function: acting as a calcium buffer, a calcium transporter, and a non-canonical calcium sensor.
  • CB's diverse roles highlight its significance in neuronal function, calcium homeostasis, and potentially other physiological processes.