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Published on: April 13, 2019
Identification of marker genes for lipid-lowering effect of a short-chain fructooligosaccharide by DNA microarray
Tomoyuki Fukasawa1, Koichiro Murashima, Tomoko Nemoto
1Food and Health R&D Laboratories, Meiji Seika Kaisha, Ltd., Chiyoda, Sakado-shi, Saitama, Japan. tomoyuki_fukasawa@meiji.co.jp
Abstract:
Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p < .05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.
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