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Updated: Aug 17, 2026

Multi-unit Recording Methods to Characterize Neural Activity in the Locust (Schistocerca Americana) Olfactory Circuits
Published on: January 25, 2013
Histamine H1-receptor-like binding sites in the locust nervous tissue
1Universität Hamburg, Zoologisches Institut, F.R.G.
Abstract:
The histamine H1-receptor-like binding sites in the nervous tissue of the locust Locusta migratoria were investigated with a conventional radio-receptor assay using [3H]mianserin as the radio ligand. Binding of [3H]mianserin to the binding site is sensitive to proteases and heat treatment. It shows the characteristics of ligand-receptor interactions. The single binding site has high affinity for mianserin (KD = 7.05 nM) and is present in high concentrations (Bmax = 1.53 pmol/mg) in the whole nervous tissue. All tested antihistamines have a high affinity for the binding site, which suggests that it represents an insect histamine receptor. Nevertheless, it shows its peculiarities distinguishing it from vertebrate histamine H1-receptors.
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