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Updated: May 23, 2026
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A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
Published on: June 30, 2021
Morphological analysis of zebrafish embryo tails
Jianfeng Lu1, Tao Wu, Lei Cheng
1School of Computer Science and Technology, Nanjing University of Science and Technology, Nanjing, China. lujf@njust.edu.cn
Summary
This study presents an automated pipeline for quantifying zebrafish embryo tail curvedness. The method achieves accuracy comparable to manual analysis, enhancing high-content screening.
Area of Science:
- Developmental biology
- Image analysis
- Computational biology
Background:
- Quantitative analysis of image-based phenotypes is crucial for zebrafish studies and high-content screening.
- Accurate measurement of phenotypic traits like tail curvedness is essential for understanding developmental processes.
Purpose of the Study:
- To develop and validate an automatic image analysis pipeline for quantifying zebrafish embryo tail curvedness.
- To compare the performance of different curvedness measures for skeletal analysis.
Main Methods:
- Zebrafish embryo extraction using the active contour model.
- Skeletonization of the embryo tail via the principal graph algorithm.
- Design and comparison of multiple tail skeleton curvedness measures.
Main Results:
- The proposed automatic pipeline effectively quantifies zebrafish embryo tail curvedness.
- Joint analysis of tail shape and relative position yields superior performance.
- The automated method demonstrates comparable accuracy to manual analysis.
Conclusions:
- The developed pipeline offers a reliable and efficient tool for zebrafish tail curvedness quantification.
- This automated approach can significantly benefit high-content screening and phenotypic analysis in zebrafish research.
