Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanisms of ubiquitylation of the mitotic regulatory protein Cdc20.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Evaluation of dosimetric consistency between pre- and postoperative CT-guided noncoplanar template-assisted ¹²⁵I seed implantation in recurrent and metastatic thoracic tumors: a retrospective study.

PeerJ·2026
Same author

Clinicopathological and Immunohistochemical Analysis of Recurrent Endometrial Carcinomas: A Retrospective Study from an Indian Cohort.

Journal of mid-life health·2026
Same author

Models for the architecture of the human inner kinetochore on centromeric α-satellite CENP-A nucleosome arrays.

Nature communications·2026
Same author

Canine Olfaction Combined With Bayesian Modeling for Multicancer Detection From Breath Samples: A Phase II Study in India.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026
Same author

Assembly and phosphoregulatory mechanisms of the budding yeast outer kinetochore KMN complex.

The Journal of cell biology·2026
Same journal

Daily briefing: How cooperation built the world.

Nature·2026
Same journal

Deep-sea oddities and boatloads of other new species - June's best science images.

Nature·2026
Same journal

From cloning to gene-editing: the enduring legacy of Dolly the sheep.

Nature·2026
Same journal

Time to give hydration breaks the red card? What science says about keeping cool.

Nature·2026
Same journal

Universities are relying on AI-detection software to catch cheating. How well do the programs work?

Nature·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Structure of the mitotic checkpoint complex.

William C H Chao1, Kiran Kulkarni, Ziguo Zhang

  • 1Division of Structural Biology, Institute of Cancer Research, Chester Beatty Laboratories, 237 Fulham Road, London, SW3 6JB, UK.

Nature
|March 23, 2012
PubMed
Summary
This summary is machine-generated.

The spindle assembly checkpoint (SAC) ensures genome stability by inhibiting the anaphase-promoting complex/cyclosome (APC/C). This study reveals the molecular mechanisms of SAC regulation by the mitotic checkpoint complex (MCC), detailing how MCC assembly and APC/C inhibition are controlled.

More Related Videos

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Related Experiment Videos

Last Updated: May 23, 2026

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The spindle assembly checkpoint (SAC) is crucial for maintaining genome stability during cell division.
  • The mitotic checkpoint complex (MCC) enforces the SAC by inhibiting the anaphase-promoting complex/cyclosome (APC/C).
  • Unattached chromosomes trigger MCC assembly, which then targets APC/C, a key regulator of chromosome segregation.

Purpose of the Study:

  • To elucidate the molecular basis of MCC-mediated APC/C inhibition.
  • To understand the regulatory mechanisms governing MCC assembly.
  • To reveal how APC/C inhibition is linked to co-activator degron recognition.

Main Methods:

  • X-ray crystallography of the Schizosaccharomyces pombe MCC.
  • Structural analysis of MCC complex formation and APC/C interaction.
  • Biochemical assays to study MCC assembly and inhibition dynamics.

Main Results:

  • The crystal structure reveals how MCC inhibits APC/C by obstructing Cdc20 binding sites and disrupting APC/C-Cdc20 complex formation.
  • Closed-conformational Mad2 (C-Mad2) stabilizes the MCC by facilitating Mad3 KEN-box binding to Cdc20.
  • p31(comet) competes with Mad3 for the C-Mad2 interface, thereby antagonizing the SAC by disrupting MCC assembly.

Conclusions:

  • The study provides a detailed molecular understanding of MCC function in SAC regulation.
  • APC/C inhibition is directly coupled to the recognition of degrons by MCC co-activators.
  • These findings offer insights into the intricate mechanisms ensuring accurate chromosome segregation and genome stability.