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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400 keV in...

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Related Experiment Video

Updated: May 23, 2026

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

Electron tomography at 2.4-ångström resolution.

M C Scott1, Chien-Chun Chen, Matthew Mecklenburg

  • 1Department of Physics and Astronomy and California NanoSystems Institute, University of California, Los Angeles, California 90095, USA.

Nature
|March 23, 2012
PubMed
Summary

A new general electron tomography method enables atomic-scale 3D imaging of nanomaterials without prior structural assumptions. This technique achieved 2.4-ångström resolution for a gold nanoparticle, revealing its detailed morphology and lattice structure.

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Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
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08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Area of Science:

  • Materials Science
  • Nanoscience
  • Biology
  • Electron Microscopy

Background:

  • Transmission electron microscopy (TEM) offers powerful imaging for materials science, nanoscience, and biology.
  • Aberration-corrected electron lenses significantly enhance TEM resolution, achieving below 0.5 ångströms.
  • Electron tomography is crucial for 3D structure determination of thin samples, with current cubic-nanometre resolution.

Purpose of the Study:

  • To demonstrate a general electron tomography method for atomic-scale resolution 3D imaging.
  • To overcome limitations of discrete tomography, which requires prior knowledge of lattice structure.
  • To apply the method to a gold nanoparticle and characterize its structure.

Main Methods:

  • Combined scanning transmission electron microscopy (STEM) with a novel projection alignment and tomographic reconstruction method.
  • Developed a general electron tomography approach without initial assumptions about sample structure.
  • Achieved atomic-scale resolution imaging.

Main Results:

  • Determined the 3D structure of a ~10 nm gold nanoparticle at 2.4 ångström resolution.
  • Observed individual atoms in specific regions and identified several grains in three dimensions.
  • Revealed 3D surface morphology and internal lattice structure consistent with a distorted icosahedral multiply twinned particle.

Conclusions:

  • The demonstrated general electron tomography method achieves atomic-scale resolution for nanomaterials.
  • This technique provides insights into nanoparticle structure without prior structural assumptions.
  • The method has potential applications in various tomography fields for improved resolution and image quality.