Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Lines

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...

Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

Bioreactor Controls-III

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A 3D Human Bone and Bone Marrow-on-a-Chip Model for In Vitro Bone Remodeling and Immune Cell Maintenance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Differential biomarker expression of blood and lymphatic vasculature in multi-organ-chips.

Scientific reports·2025

Same author

Biology-inspired dynamic microphysiological system approaches to revolutionize basic research, healthcare and animal welfare.

ALTEX·2025

Same author

Hepatotoxic assessment in a microphysiological system: Simulation of the drug absorption and toxic process after an overdosed acetaminophen on intestinal-liver-on-chip.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association·2024

Same author

Normoglycemia and physiological cortisone level maintain glucose homeostasis in a pancreas-liver microphysiological system.

Communications biology·2024

Same author

Application of a skin and liver Chip2 microphysiological model to investigate the route-dependent toxicokinetics and toxicodynamics of consumer-relevant doses of genistein.

Journal of applied toxicology : JAT·2023

Search research articles

Related Experiment Video

Updated: May 23, 2026

BioMEMS and Cellular Biology: Perspectives and Applications

BioMEMS and Cellular Biology: Perspectives and Applications

Published on: October 1, 2007

Trends in cell culture technology.

¹ProBioGen AG, Berlin, Germany. uwe.marx@probiogen.de

Advances in Experimental Medicine and Biology

|March 23, 2012

Summary

Advanced bioreactor systems offer new ways to test chemical and drug safety. Miniaturization and standardized tissues are key challenges for high-throughput substance testing using cell culture technology.

Area of Science:

Biotechnology
Toxicology
Cell Culture Technology

Background:

Dynamic macroscale bioreactor systems represent a significant advancement in cell culture.
A critical gap exists in predictive safety and mechanism-of-action testing for various substances.
Current cell culture limitations hinder the translation of bioreactor technology for high-throughput screening.

Purpose of the Study:

To explore recent developments in cell culture technology for predictive substance testing.
To present a novel multi-micro-organ bioreactor concept.
To identify pathways for overcoming current technological barriers.

Main Methods:

Review of recent advancements in bioreactor systems and cell culture.
Illustration of a multi-micro-organ bioreactor design.

More Related Videos

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Published on: January 18, 2012

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Related Experiment Videos

Last Updated: May 23, 2026

BioMEMS and Cellular Biology: Perspectives and Applications

BioMEMS and Cellular Biology: Perspectives and Applications

Published on: October 1, 2007

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model

Published on: January 18, 2012

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Scale-Up of Mammalian Cell Culture using a New Multilayered Flask

Published on: December 5, 2011

Analysis of challenges in miniaturization and tissue standardization.

Main Results:

Dynamic macroscale bioreactors offer potential for improved substance testing.
Miniaturization to microliter scale and standardized human tissue supply are identified as major hurdles.
A multi-micro-organ bioreactor concept is proposed to address these challenges.

Conclusions:

Bioreactor technology holds promise for predictive toxicology and pharmaceutical testing.
Overcoming miniaturization and tissue supply challenges is crucial for realizing high-throughput applications.
Further development is needed to bridge the gap between current technology and practical predictive testing solutions.