Related Experiment Video

Updated: May 23, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

MHC I-associated peptides preferentially derive from transcripts bearing miRNA response elements

Diana Paola Granados1, Wafaa Yahyaoui, Céline M Laumont

  • 1Institute for Research in Immunology and Cancer, Université deMontréal, Montréal, QC, Canada.

Blood
|March 23, 2012
PubMed

Insights

MHC I-associated peptides (MIPs) are generated from defective ribosomal products (DRiPs) and regulated by microRNAs (miRNAs). This study reveals how transcript abundance and DRiP rate influence MIP presentation, offering insights into cellular immune responses.

Area of Science:

  • Immunology
  • Molecular Biology
  • Proteomics

Background:

  • MHC I-associated peptides (MIPs) are crucial for immune surveillance and homeostasis.
  • MIPs primarily originate from defective ribosomal products (DRiPs), whose nature is not fully understood.
  • Understanding MIP biogenesis is key to deciphering cellular immune responses.

Purpose of the Study:

  • To investigate the structure and biogenesis of MIPs presented by HLA-A and HLA-B molecules.
  • To elucidate the relationship between transcript characteristics and MIP generation.
  • To explore the role of microRNAs (miRNAs) in regulating MIP presentation.

Main Methods:

  • High-throughput proteomic and transcriptomic analyses were employed.
  • Analysis was performed on human EBV-infected B lymphocytes from four patients.
  • Investigated peptide-MHC interactions and transcriptomic profiles.

Main Results:

  • Distinct MIPs were observed in HLA-different individuals, originating from functionally related proteins.
  • MIP repertoire showed no bias towards conserved or polymorphic sequences, favoring abundant transcripts.
  • MIPs were preferentially derived from transcripts with miRNA response elements, regulated by similar miRNAs across individuals.

Conclusions:

  • MIP generation is influenced by transcript abundance and DRiP rate.
  • MicroRNAs play a significant role in regulating the generation of MIPs from specific transcripts.
  • This provides a model for understanding how cellular immune signatures are established.

More Related Videos

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

mirMachine: A One-Stop Shop for Plant miRNA Annotation06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Processing Pathways01:31

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...