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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 23, 2026

Sample Preparation for Mass Cytometry Analysis
06:28

Sample Preparation for Mass Cytometry Analysis

Published on: April 29, 2017

Cluster cytometry for high-capacity bioanalysis.

Bruce S Edwards1, Jingshu Zhu, Jun Chen

  • 1Department of Pathology, University of New Mexico, Albuquerque, New Mexico, USA. bedwards@salud.unm.edu

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|March 23, 2012
PubMed
Summary
This summary is machine-generated.

A new Cluster Cytometer platform enhances flow cytometry for high-throughput screening (HTS). This system analyzes hundreds of thousands of samples efficiently, boosting throughput and enabling large-scale biological investigations.

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Last Updated: May 23, 2026

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Flow cytometry excels at single-cell analysis but has limitations in multi-experiment throughput.
  • High-throughput screening (HTS) requires analyzing vast numbers of samples efficiently.
  • Advancements like HyperCyt sampling technology are improving flow cytometry's capacity for HTS.

Purpose of the Study:

  • To develop and assess a HyperCyt-linked Cluster Cytometer platform for enhanced HTS.
  • To address technical challenges associated with analyzing small-volume samples in high-density plates.
  • To improve the efficiency and automation compatibility of flow cytometry for large-scale biological studies.

Main Methods:

  • A network of flow cytometers (Cluster Cytometer) was linked with HyperCyt sampling technology.
  • Samples were analyzed in high-density, 1,536-well plates with assay volumes of 10 microL or less.
  • Performance was assessed using well-characterized cell- and microsphere-based HTS assays.

Main Results:

  • The Cluster Cytometer platform demonstrated practical practicality for analyzing hundreds-of-thousands of samples.
  • Technical issues such as reagent mixing and cell suspension in small wells were addressed.
  • The platform boosted sample throughput fourfold and showed potential for reanalysis if needed.

Conclusions:

  • The HyperCyt-linked Cluster Cytometer platform significantly enhances flow cytometry's capability for HTS.
  • This technology is well-positioned to integrate with emerging suspension array and cell barcoding technologies.
  • Cluster cytometry offers a practical and productive approach for HTS and large-scale biological complexity investigations.