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Updated: May 23, 2026

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

Virus-induced Gene Silencing (VIGS) in Nicotiana benthamiana and Tomato

Published on: June 10, 2009

Virus-derived gene expression and RNA interference vector for grapevine.

Elizabeth G Kurth¹, Valera V Peremyslov, Alexey I Prokhnevsky

¹Department of Botany and Plant Pathology, Oregon State University, Corvallis, Oregon, USA.

Journal of Virology

|March 23, 2012

Summary

This summary is machine-generated.

Scientists developed a novel RNA virus vector to enhance grapevine traits without genetic modification. This technology aids in functional genomics and disease control, overcoming challenges in traditional agriculture.

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Area of Science:

Plant Biotechnology
Molecular Biology
Agricultural Science

Background:

Traditional grapevine (Vitis vinifera) improvement faces limitations due to variety adherence, genetic modification recalcitrance, and public GMO concerns.
Developing new grapevine varieties with improved agricultural and wine-making qualities is crucial for the industry.

Purpose of the Study:

To create a novel RNA virus-based vector for introducing desired traits into grapevines.
To enable trait introduction without heritable genomic modifications, addressing public and technical barriers.
To leverage the vector for functional genomics and disease control applications.

Main Methods:

Development of an RNA virus vector expressing recombinant proteins in grapevine phloem tissue.
Utilizing virus-induced gene silencing (VIGS) for RNA interference (RNAi) and endogenous gene regulation.
Assessing vector's genetic capacity, stability, and implementation speed.

Main Results:

The vector successfully introduces desired traits into grapevine without altering the genome.
It facilitates expression of proteins in phloem, impacting sugar transport.
The vector demonstrates potent RNAi capability for gene regulation and potential for VIGS applications.

Conclusions:

The RNA virus vector offers a non-heritable method for grapevine genetic improvement.
It presents significant applications in grapevine functional genomics and developing novel disease/pest control strategies.
This technology overcomes key hurdles in grapevine biotechnology, offering rapid and stable trait introduction.