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Updated: May 23, 2026
Rapid Diagnosis of Avian Influenza Virus in Wild Birds: Use of a Portable rRT-PCR and Freeze-dried Reagents in the Field
Published on: August 2, 2011
The survey of wild birds for West Nile virus in Poland
J S Niczyporuk1, E Samorek-Salamonowicz, W Kozdruń Z Mizak
1National Veterinary Research Institute Pulawy, (NVRI) Department of Poultry Viral Diseases, Al. Partyzantow 57, 24-100 Pulawy, Poland. jowita.niczyporuk@piwet.pulawy.pl
Abstract:
Two thousand one hundred and forty birds belonging to 39 different species from different locations in Poland were examined. The study has taken place from the early spring till late autumn 2007-2010 when the activity of the mosquitoes was the highest. The brain samples were taken from the birds and whole cellular RNA was isolated, then the RT-PCR and NRT-PCR were performed to detect the presence of West Nile virus (WNV). The obtained results were confirmed by the commercial WNV Kit. No genetic material of WNV was found in the examined samples.
