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Updated: May 23, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
β-adrenoreceptor activation in brain, lung and adipose tissue, measured by microdialysis in pig
1Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. elham.rostami@ki.se
Purpose:
The aim of this study is to investigate the effect of local activation of β-adrenoreceptor by Isoprenaline on metabolism in brain, fat and lung measured by microdialysis.
Methods:
We used 8 healthy pigs under general anaesthesia and placed microdialysis catheters in brain, fat, lung and artery. We performed a direct measurement of glucose, lactate, pyruvate and glycerol. The stimulation was performed by one-hour infusion of Isoprenaline, a β-adrenoreceptor agonist.
Results:
The infusion of isoprenaline did not affect the glucose in any tissue. The levels of lactate (p=0.008) and pyruvate (p=0.011) decreased significantly in lung after isoprenaline infusion. There was a significant increase in L/P ratio in fat tissue (p=0.001) while no significant changes could be found in brain (p=0.086) and lung (p=0.679). The most pronounced and significant change was observed in glycerol in fat (p<0.001) that increased by 95%.
Conclusion:
The prominent increase in glycerol in fat proved to be a good measure of β-adrenoreceptor activation and a measure of lipolysis. This can be used to online monitor β-adrenoreceptor activation by glycerol measurement in patients.
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