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Establishing In Situ Closed Circuit Perfusion of Lower Abdominal Organs and Hind Limbs in Mice

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An isolated tumor perfusion model in mice.

Annique M M J Duyverman¹, Mitsutomo Kohno, Sylvie Roberge

¹Edwin L. Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital (MGH) and Harvard Medical School, Boston, Massachusetts, USA.

Nature Protocols

|March 24, 2012

Summary

This study introduces a novel perfusion model to investigate how stromal cells contribute to cancer cell shedding and metastasis. The model allows tracking of GFP-labeled stromal cells in circulating tumor fragments.

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Area of Science:

Oncology
Cell Biology
Cancer Research

Background:

Stromal cells are integral components of the tumor microenvironment.
Stromal cells play a significant role in various stages of cancer metastasis.
Previous research has extensively characterized the general role of stromal cells.

Purpose of the Study:

To present a detailed protocol for an isolated tumor perfusion model.
To enable the study of cancer and stromal cell shedding.
To facilitate research on therapies targeting tumor cell or fragment shedding.

Main Methods:

Development of an isolated tumor perfusion model.
Utilizing a microenvironment where stromal cells ubiquitously express Green Fluorescent Protein (GFP).

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Orthotopic or ectopic implantation of primary tumors for study.

Main Results:

The model allows for the detection and study of stromal cells within circulating tumor fragments in mice.
Demonstrates the capacity to track GFP-labeled stromal cells in circulation.
The methodology supports investigation into the role of stromal cells in the metastatic cascade.

Conclusions:

This perfusion model provides a valuable tool for studying stromal cell involvement in cancer cell shedding.
It facilitates research into the mechanisms of metastasis and the development of anti-metastatic therapies.
The model enables detailed analysis of circulating tumor fragments and their associated stromal components.