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Updated: May 23, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Published on: July 5, 2016

Quantitative phase microscopy using dual-plane in-line digital holography.

Bhargab Das1, Chandra S Yelleswarapu, D V G L N Rao

  • 1Physics Department, University of Massachusetts, Boston, Massachusetts 02125, USA. bhargab.das@gmail.com

Applied Optics
|March 24, 2012
PubMed
Summary

This study enhances quantitative phase imaging using dual-plane digital holographic microscopy. The technique accurately reconstructs object details by eliminating unwanted light waves for improved imaging of cells and materials.

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy

Background:

  • Quantitative phase imaging (QPI) is crucial for label-free cell analysis.
  • Digital holographic microscopy (DHM) offers high-resolution imaging capabilities.
  • In-line holographic setups can suffer from zero-order and twin-image noise.

Purpose of the Study:

  • To theoretically evaluate and experimentally validate a dual-plane in-line digital holographic microscopy technique for QPI.
  • To demonstrate the removal of zero-order and twin-image noise for enhanced image reconstruction.
  • To showcase the technique's potential in imaging biological samples like human muscle cells.

Main Methods:

  • Utilizing a dual-plane in-line digital holographic microscopy setup.
  • Recording two interferograms at slightly different focal planes.
  • Applying numerical reconstruction algorithms, including average intensity subtraction and Fourier domain processing for noise elimination.

Main Results:

  • Successful elimination of zero-order diffracted waves via intensity subtraction.
  • Effective removal of twin-image diffracted waves using Fourier domain processing.
  • Demonstrated accurate quantitative phase reconstruction of controlled objects and human muscle cells.

Conclusions:

  • The dual-plane in-line DHM technique provides robust quantitative phase imaging.
  • The noise reduction methods significantly improve image fidelity.
  • This technique shows great promise for advanced biological and materials science imaging applications.