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Updated: May 23, 2026
Assessing Whole-Body Lipid-Handling Capacity in Mice
Published on: November 24, 2020
Hepatic lipid composition differs between ob/ob and ob/+ control mice as determined by using in vivo localized proton
Qiong Ye1, Carsten Friedrich Danzer, Alexander Fuchs
1Institute for Biomedical Engineering UZH/ETH, University and ETH Zurich, HIT E22.4, Wolfgang-Pauli-Strasse 27, 8093, Zurich, Switzerland.
Object:
Hepatic lipid accumulation is associated with nonalcoholic fatty liver disease, and the metabolic syndrome constitutes an increasing medical problem. In vivo proton magnetic resonance spectroscopy ((1)H MRS) allows the assessment of hepatic lipid levels noninvasively and also yields information on the fat composition due to its high spectral resolution.
Materials And Methods:
We applied (1)H MRS at 9.4T to study lipid content and composition in eight leptin-deficient ob/ob mice as a model of obesity and in four lean ob/+ control mice at 24 weeks of age. PRESS sequence was used. For accurate estimation of signal intensity, differences in relaxation behavior of individual signals were accounted for each mouse individually. Also, in order to minimize spectral degrading due to motion artifacts, respiration gating was applied.
Results:
Significant differences between ob/ob and ob/+ control mice were found in both lipid content and composition. The mean chain length was found to be significantly longer in ob/ob mice with a higher fraction of monounsaturated lipids.
Conclusion:
(1)H MRS enables accurate assessment in hepatic lipids in mice, which is attractive for mechanistic studies of altered metabolism given the large number of genetically engineered mouse models available.
