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Updated: May 23, 2026

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test
Published on: April 9, 2012
Molecular detection of rifabutin-susceptible Mycobacterium tuberculosis
Huang-Yao Chen1, Ming-Chih Yu, Wei-Lun Huang
1Reference Laboratory of Mycobacteriology, Research and Diagnostic Center, Centers for Disease Control, Taipei, Taiwan.
Abstract:
Rapid assays are still needed to detect rifabutin (RFB) susceptibility for proper tuberculosis treatment. To assess the use of the GenoType MTBDRplus assay and subsequent rpoB gene sequencing on detection of RFB susceptibility, we analyzed 800 multidrug-resistant Mycobacterium tuberculosis isolates, and 13% (104/800) were RFB susceptible. Of the 104 RFB-susceptible isolates, 71 (68.3%) isolates were rapidly identified using two molecular assays, while the remaining isolates could be determined using conventional drug-susceptibility testing according to the clinician's decision.
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