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Related Concept Videos

Oogenesis01:22

Oogenesis

Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...
Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

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Related Experiment Video

Updated: May 23, 2026

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

MARF1 regulates essential oogenic processes in mice.

You-Qiang Su1, Koji Sugiura, Fengyun Sun

  • 1The Jackson Laboratory, Bar Harbor, ME 04609, USA.

Science (New York, N.Y.)
|March 24, 2012
PubMed
Summary
This summary is machine-generated.

Meiosis arrest female 1 (MARF1) is crucial for female fertility. Its absence causes infertility by disrupting oocyte maturation, increasing retrotransposons, and leading to DNA damage.

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Last Updated: May 23, 2026

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Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Oocyte development

Background:

  • Fertilization-competent oocytes require coordinated meiosis, cytoplasmic maturation, and genomic stability.
  • The role of Meiosis Arrest Female 1 (MARF1) in mammalian oogenesis is not fully understood.

Purpose of the Study:

  • To investigate the function of MARF1 in mammalian oocyte development and female fertility.
  • To elucidate the molecular mechanisms underlying infertility caused by Marf1 mutations.

Main Methods:

  • Analysis of Marf1 mutant mice to assess female fertility.
  • Transcriptomic analysis to identify differentially expressed genes.
  • Assessment of retrotransposon expression and DNA integrity in oocytes.

Main Results:

  • Marf1 mutations lead to female infertility with defective oocyte maturation and meiotic arrest.
  • Mutant oocytes exhibit up-regulation of specific transcripts, including protein phosphatase 2 catalytic subunit (PPP2CB).
  • Increased expression of retrotransposons (Iap, Line1) and elevated DNA double-strand breaks were observed in Marf1 mutant oocytes.

Conclusions:

  • MARF1 is essential for regulating oocyte maturation, genomic integrity, and female fertility.
  • MARF1 functions by suppressing specific transcripts, including PPP2CB, and controlling retrotransposon activity.
  • Disruption of MARF1 function has significant implications for reproductive health and offspring development.