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Updated: May 23, 2026
Isolation, Expansion, and Nucleofection of Neural Stem Cells from Adult Murine Subventricular Zone
Published on: June 14, 2024
Neurons and stromal stem cells as targets for polycation-mediated transfection
A V Shakhbazau1, D G Shcharbin, N V Goncharova
1Department of Clinical Neurobiology, University of Calgary, Canada. shakhbazau@gmail.com
Abstract:
Expression of transgenes in neurons and stromal/mesenchymal stem cells (MSC) can greatly enhance their therapeutic potential. In transfection experiments, we studied properties of linear and branched (dendrimers) polycations as transgene delivery vehicles. Linear polyethyleneimine transfected neurons, but was ineffective in MSC. Polyamidoamine dendrimers showed greater transfection efficiency and mean GFP fluorescence intensity compared to phosphorus dendrimers of the same (4th) generation. Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher.
