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Plasma cyclic GMP: response to cholinergic agents.

M Honma, M Ui

    European Journal of Pharmacology
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Cholinergic drugs rapidly increase plasma cyclic GMP in rats, with cyclic AMP levels rising subsequently. Atropine blocks this effect, suggesting plasma cyclic GMP indicates cholinergic activity.

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    Area of Science:

    • Pharmacology
    • Neuroscience
    • Biochemistry

    Background:

    • Cholinergic signaling plays a crucial role in various physiological processes.
    • Cyclic nucleotides, such as cyclic GMP (cGMP) and cyclic AMP (cAMP), act as important second messengers.
    • Understanding the dynamic changes in these signaling molecules in response to cholinergic stimulation is vital.

    Purpose of the Study:

    • To investigate the effect of cholinergic agents on plasma cyclic GMP and cyclic AMP concentrations in rats.
    • To determine the primary cyclic nucleotide affected by cholinergic drugs.
    • To assess the role of atropine in modulating cholinergic-induced cyclic nucleotide changes.

    Main Methods:

    • Subcutaneous injections of cholinergic agents (carbachol, methacholine, bethanechol, acetylcholine with physostigmine) were administered to fasted rats.

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  • Plasma concentrations of cyclic GMP and cyclic AMP were measured at various time points post-injection.
  • Atropine was used to evaluate its effect on baseline and drug-induced cyclic nucleotide levels.

    • Main Results:

    • Cholinergic agents significantly increased plasma cyclic GMP levels, peaking within 5-10 minutes.
    • Acetylcholine induced a significant cyclic GMP increase only when co-administered with physostigmine.
    • Cyclic AMP levels increased secondary to cyclic GMP elevation, indicating cGMP as the primary target.
    • Atropine abolished the drug-induced rise in cyclic GMP and reduced baseline levels.

    Conclusions:

    • Plasma cyclic GMP concentration serves as a reliable indicator of cholinergic activity in rats.
    • Cholinergic drugs primarily elevate cyclic GMP, with cyclic AMP changes being secondary.
    • Atropine effectively antagonizes cholinergic effects on cyclic GMP.