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Related Experiment Video

Updated: May 23, 2026

Total Internal Reflection Absorption Spectroscopy (TIRAS) for the Detection of Solvated Electrons at a Plasma-liquid Interface

Total Internal Reflection Absorption Spectroscopy (TIRAS) for the Detection of Solvated Electrons at a Plasma-liquid Interface

Published on: January 24, 2018

Spatially resolved scattering correlation spectroscopy using a total internal reflection configuration.

Heng Liu¹, Chaoqing Dong, Xiangyi Huang

¹College of Chemistry & Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiaotong University, Shanghai, People's Republic of China.

Analytical Chemistry

|March 27, 2012

Summary

This summary is machine-generated.

We developed spatially resolved scattering correlation spectroscopy (SRSCS) for single nanoparticle analysis. This novel method offers multiplexing and spatial resolution, enabling detailed studies of complex biological systems.

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Area of Science:

Nanotechnology
Spectroscopy
Biophysics

Background:

Fluorescence correlation spectroscopy (FCS) is limited by photobleaching.
Single nanoparticle analysis requires sensitive and high-resolution techniques.

Purpose of the Study:

To introduce a novel single particle method, spatially resolved scattering correlation spectroscopy (SRSCS).
To establish and validate the SRSCS system for nanoparticle detection and imaging.
To explore the potential of SRSCS for studying heterogeneous systems.

Main Methods:

Utilizing total internal reflection (TIR) configuration and resonance light scattering (RLS) of silver nanoparticles (AgNPs).
Employing a millimeter-scale hole for scattering light separation and an electron multiplying charge-coupled device (EMCCD) for detection.
Developing an SRSCS model based on FCS principles and investigating influencing factors.

Main Results:

Successfully established a highly sensitive SRSCS system.
Demonstrated effective detection and imaging of single AgNPs in solution.
Validated the SRSCS model with calibration experiments showing good agreement.

Conclusions:

SRSCS is a novel, sensitive, and spatially resolved single particle analysis technique.
The method is multiplexing, free of photobleaching, and suitable for heterogeneous systems.
SRSCS holds promise for studying biological processes like protein motion on cell membranes.