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Updated: May 23, 2026
Culturing Rat Sympathetic Neurons from Embryonic Superior Cervical Ganglia for Morphological and Proteomic Analysis
Published on: September 27, 2020
A proteome map of primary cultured rat Schwann cells
Mi Shen1, Yuhua Ji1,2, Shuqiang Zhang1
1Jiangsu Key Laboratory of Neuroregeneration, Nantong University, 19 Qixiu Road, Nantong, Jiangsu Province 226001, Peoples' Republic of China.
Background:
Schwann cells (SCs) are the principal glial cells of the peripheral nervous system with a wide range of biological functions. SCs play a key role in peripheral nerve regeneration and are involved in several hereditary peripheral neuropathies. The objective of this study was to gain new insight into the whole protein composition of SCs.
Results:
Two-dimensional liquid chromatography coupled with tandem mass spectrometry (2D LC-MS/MS) was performed to identify the protein expressions in primary cultured SCs of rats. We identified a total of 1,232 proteins, which were categorized into 20 functional classes. We also used quantitative real time RT-PCR and Western blot analysis to validate some of proteomics-identified proteins.
Conclusion:
We showed for the first time the proteome map of SCs. Our data could serve as a reference library to provide basic information for understanding SC biology.
