Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes01:28

Pharmacogenetics of Phase I Enzymes: Cytochrome P450 Isozymes

Cytochrome P450 (CYP450) enzymes are a superfamily of heme-containing monooxygenases that play a pivotal role in Phase I drug metabolism by catalyzing oxidation and reduction reactions.These enzymes transform lipophilic xenobiotics into more hydrophilic metabolites, facilitating subsequent Phase II conjugation and eventual excretion. The CYP450 family is classified into families (e.g., CYP1–CYP3) and subfamilies (e.g., CYP2A, CYP2C), based on amino acid sequence homology.CYP450 isoenzymes,...
Drug Metabolism: Phase I Reactions01:17

Drug Metabolism: Phase I Reactions

A phase I reaction is a biochemical process that introduces a functionally reactive polar group to a substance. This transformation predominantly occurs in the liver, facilitated by the cytochrome P450 system of hemoproteins situated in the lipophilic endoplasmic reticulum of cells. The metabolite generated through this process can have varying polarities. If it is sufficiently polar, it can be easily excreted in the urine due to its water compatibility. However, if the metabolite is nonpolar,...
Bioactivation and Tissue Toxicity01:25

Bioactivation and Tissue Toxicity

Bioactivation is a metabolic process that transforms less reactive substances into highly reactive metabolites, initiating tissue toxicity. This transformation can lead to various toxic effects, including carcinogenesis and teratogenesis. Reactive metabolites are classified into two main types: electrophiles and free radicals.Electrophiles are electron-deficient species and are produced primarily by the enzyme cytochrome P-450 during the metabolism of compounds containing carbon, nitrogen, or...
Pharmacogenetics of Drug Metabolism: Overview01:27

Pharmacogenetics of Drug Metabolism: Overview

Genetic polymorphism in drug metabolism is crucial to the inter-individual variability observed in drug responses. Drug metabolism primarily involves the chemical modification of drugs and other xenobiotics to enhance their elimination by increasing their polarity. Two main classes of enzymes mediate this biotransformation process: Phase I enzymes, primarily cytochrome P450s, catalyze oxidation and reduction reactions, while other enzymes, such as esterases, mediate hydrolysis, and Phase II...
Peroxisomes and Mitochondria01:30

Peroxisomes and Mitochondria

Peroxisomes and mitochondria are two important oxygen-utilizing organelles in eukaryotic cells. Mitochondria carry out cellular respiration—the process that converts energy from food into ATP. Peroxisomes carry out a variety of functions, primarily breaking down different substances, such as fatty acids.
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within peroxisomes...
Peroxisomes01:24

Peroxisomes

Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Mycobacterium tuberculosis Rv0132c Gene Product Mtb-FGD2 Can Act as an F<sub>420</sub>-Dependent Glucose Dehydrogenase.

Proteins·2026
Same author

Integrated structural dynamics uncover a new B<sub>12</sub> photoreceptor activation mode.

Nature·2026
Same author

Structure and Mechanism of PhdC, a Prenylated-Flavin Maturase.

Proteins·2025
Same author

Fragment-Based Development of Small Molecule Inhibitors Targeting <i>Mycobacterium tuberculosis</i> Cholesterol Metabolism.

Journal of medicinal chemistry·2025
Same author

Engineered enzymes for enantioselective nucleophilic aromatic substitutions.

Nature·2025
Same author

Fragment-based development of small molecule inhibitors targeting <i>Mycobacterium tuberculosis</i> cholesterol metabolism.

bioRxiv : the preprint server for biology·2025
Same journal

Nephronophthisis: Current clinical spectrum and molecular pathogenesis.

The FEBS journal·2026
Same journal

PDC1 deficiency results in 2-deoxyglucose sensitivity through inhibition of Pdc2 activity in yeast.

The FEBS journal·2026
Same journal

Epigenetic regulation of the hepcidin gene expression in hepatoma cells.

The FEBS journal·2026
Same journal

Loss of Ambp ameliorates steatosis progression by activating PPARα signaling in zebrafish.

The FEBS journal·2026
Same journal

Varying susceptibility of subpopulations along the epithelial-mesenchymal spectrum to undergo EMT.

The FEBS journal·2026
Same journal

ALOX15 links lipid metabolism to receptor trafficking in platelet activation.

The FEBS journal·2026
See all related articles

Related Experiment Video

Updated: May 23, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

Special issue: Cytochrome P450 structure and function: introduction.

Andrew W Munro1, David Leys

  • 1Manchester Interdisciplinary Biocentre, Faculty of Life Sciences, University of Manchester, Manchester, UK.

The FEBS Journal
|March 27, 2012
PubMed
Summary
This summary is machine-generated.

The 17th International Conference on Cytochrome P450 Biochemistry, Biophysics and Structure highlighted P450 research across diverse fields. This issue presents key findings in areas including steroid metabolism, plant biochemistry, and structural biology.

More Related Videos

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

Related Experiment Videos

Last Updated: May 23, 2026

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
09:33

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling

Published on: March 20, 2018

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
06:31

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

Published on: March 19, 2020

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
10:44

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures

Published on: March 28, 2017

Area of Science:

  • Biochemistry
  • Structural Biology
  • Biotechnology

Background:

  • The 17th International Conference on Cytochrome P450 Biochemistry, Biophysics and Structure convened in Manchester, UK.
  • This conference showcased advancements in understanding Cytochrome P450 enzymes.

Discussion:

  • The conference proceedings cover a wide spectrum of P450-related research.
  • Articles reflect the significant impact of P450 research on various scientific disciplines.

Key Insights:

  • P450 research is crucial in understanding steroid metabolism.
  • Advancements in plant biochemistry and structural biology were presented.
  • The role of P450s in biotechnology applications was explored.

Outlook:

  • Future research directions in P450 science.
  • The integration of P450 structural data with functional studies.
  • Expanding P450 applications in biotechnology and medicine.