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Collagen vitrigel scaffold for regenerative medicine of the trachea: experimental study and quantitative evaluation
Yasuhiro Tada1, Toshiaki Takezawa, Akiko Tani
1Department of Otolaryngology, School of Medicine, Fukushima Medical University, Kyoto, Japan.
Conclusion:
Our technique for tracheal regeneration using a collagen vitrigel scaffold affords a feasible approach for accelerating epithelial regeneration on the intraluminal surface of the host tracheal defect.
Objectives:
The purpose of the study was to quantitatively evaluate the effectiveness of a collagen vitrigel scaffold for the regeneration of the tracheal epithelium.
Methods:
The collagen vitrigel scaffold was developed by conjugating a collagen vitrigel membrane to a collagen sponge to promote both epithelial cell growth and mesenchymal cell infiltration. The collagen vitrigel scaffold was implanted into tracheal defects in rats as the vitrigel model, and a conventional collagen sponge was implanted as a control model. The structure of the regenerated tissue was observed and thickness of the epithelium was quantitatively evaluated by histological examination.
Results:
Histological findings showed the surface of the collagen vitrigel scaffold to be flat in comparison with that of the conventional collagen sponge. At 7, 14, and 28 days post-implantation, the average thickness of the regenerated epithelial layer in the vitrigel model group was greater than that in the control group.
