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Updated: May 23, 2026

Detection of Detergent-sensitive Interactions Between Membrane Proteins
Published on: March 7, 2018
Sequential determination of anionic-type detergents by complexation with methylene blue using dual high speed
Eiichi Kitazume1, Saki Koikawa, Lu Hui
1Faculty of Humanities and Social Science, Iwate University, Ueda, 3-18-37 Morioka, Iwate 020-8550, Japan. kitazume@iwate-u.ac.jp
None:
A new dual high-speed counter-current chromatographic system using organic extraction phase and aqueous mobile phase containing methylene blue was applied to the analysis of anionic-type detergents. After selecting appropriate conditions such as flow rate of each mobile phase and sample volume, the new system was successfully applied to the analysis of anionic detergent in river water. As all the analytical procedures can be made in a closed system, the method has no health hazard. The present method is safe, precise, and highly sensitive, and can be applied for sequential determination of multiple samples in a short analysis time.
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