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[Effect of arctiin on mouse podocyte epithelial-mesenchymal transition induced by advanced oxidation protein
Jun Zhang1, Ting-Ting Guo, Lei Yang
1Department of Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Objective:
To study the effect of arctiin on mouse podocyte epithelial-mesenchymal transition (EMT) induced by advanced oxidation protein products (AOPP).
Methods:
Mouse podocytes were stimulated by 200 µg/ml AOPP for 24 h in the presence of 50, 100, 200, and 400 µmol/L arctiin. The expressions of α-smooth muscle actin, Grp78 and CHOP were detected using Western blotting.
Result:
The expressions of α-SMA, Grp78 and CHOP were inhibited by arctiin, showing a dose-dependent effect within a given range of arctiin concentration.
Conclusion:
AOPP causes endoplasmic reticulum stress to induce EMT of mouse podocytes, and arctiin can decrease EMT by alleviating the stress. This finding sheds light on a new scope of research of renal fibrosis.
