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Related Concept Videos

Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Related Experiment Video

Updated: May 23, 2026

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue
08:52

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue

Published on: July 28, 2018

[Adipose tissue sample preparation for scanning electron microscopy].

Fang Cui1, Chao Liu, Jian-Min Yang

  • 1Laboratory Center of Electron Microscopy, Hebei Medical University, Shijiazhuang 050017, China. fanghua1980@hotmail.com

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|March 27, 2012
PubMed
Summary

This study presents an improved method for preparing adipose tissue for scanning electron microscopy (SEM). Enhanced sample stabilization and dehydration yield high-quality SEM images of fat tissue.

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Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue
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Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue

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Related Experiment Videos

Last Updated: May 23, 2026

Adipo-Clear: A Tissue Clearing Method for Three-Dimensional Imaging of Adipose Tissue
08:52

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Published on: July 28, 2018

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
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Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue
06:50

Dissection, Histological Processing, and Gene Expression Analysis of Murine Supraclavicular Brown Adipose Tissue

Published on: March 29, 2024

Area of Science:

  • Biochemistry
  • Microscopy
  • Cell Biology

Context:

  • Adipose tissue sample preparation for scanning electron microscopy (SEM) is challenging.
  • Conventional methods often yield unsatisfactory results due to tissue complexity.

Purpose:

  • To develop an optimized protocol for preparing adipose tissue for SEM.
  • To improve the preservation of cellular structures in adipose tissue.

Summary:

  • A novel sample preparation strategy was employed, involving osmium tetroxide postfixation to stabilize lipids and proteins.
  • Extended dehydration times were implemented to ensure complete solvent exchange.
  • This method successfully achieved high-quality SEM imaging of adipose tissue.

Impact:

  • Provides a reliable technique for detailed SEM analysis of adipose tissue.
  • Facilitates further research into the morphology and ultrastructure of fat cells.
  • Enhances the understanding of adipose tissue in various biological contexts.