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Carbonate-dissolving bacteria from 'miliolite', a bioclastic limestone, from Gopnath, Gujarat, Western India
Gangavarapu Subrahmanyam1, Ravi Vaghela, Nilesh Pinakinprasad Bhatt
1Department of Microbiology & Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara-390002, Gujarat, India.
Abstract:
In the present investigation, the abundance and molecular phylogeny of part of the culturable bacterial population involved in the dissolution of "miliolite", a bioclastic limestone, from Gopnath, India, was studied. Carbonate-dissolving bacteria were isolated, enumerated and screened for their ability to dissolve miliolite. Amplified ribosomal DNA restriction analysis (ARDRA) indicated 14 operational taxonomic units (OTUs) to be distributed in 5 different clades at a similarity coefficient of 0.85. Then, 16S rRNA sequence analysis helped to decipher that the majority of carbonate-dissolving bacteria were affiliated to phyla Firmicutes (Families Bacillaceae and Staphylococcaceae) and Actinobacteria (Family Promicromonosporaceae) indicating their role in miliolite weathering.
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